The response of Na+/K+ -ATPase of human erythrocytes to green laser light treatment
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15910177
DOI
10.33549/physiolres.930711
PII: 711
Knihovny.cz E-resources
- MeSH
- Color MeSH
- Erythrocytes radiation effects MeSH
- Fluorescent Dyes MeSH
- Low-Level Light Therapy MeSH
- Lasers * MeSH
- Humans MeSH
- Pyrimidinones MeSH
- Sodium-Potassium-Exchanging ATPase radiation effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fluorescent Dyes MeSH
- merocyanine dye MeSH Browser
- Pyrimidinones MeSH
- Sodium-Potassium-Exchanging ATPase MeSH
The objective of this study was to investigate the response of Na(+)/K(+)-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 J.cm(-2) and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medium with 2 mM ATP for 30 min. Activity of ATPase was determined colorimetrically by measuring the colored reaction product of liberated inorganic phosphate and malachite green at 640 nm. Contribution of Na(+)/K(+)-ATPase to overall phosphate production was determined using ouabain. A positive effect of green laser light on Na(+)/K(+)-ATPase activity was observed. The dependence of enzymatically liberated inorganic phosphate on light fluence showed a linear correlation (R(2)=0.96, P=0.0005) for all fluences applied (9.5-63.3 J.cm(-2)). On the other hand, MC 540-mediated phototreatment caused a suppression of enzyme activity.
References provided by Crossref.org
Tissue-specific protective properties of lithium: comparison of rat kidney, erythrocytes and brain
