The response of Na+/K+ -ATPase of human erythrocytes to green laser light treatment
Jazyk angličtina Země Česko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15910177
DOI
10.33549/physiolres.930711
PII: 711
Knihovny.cz E-zdroje
- MeSH
- barva MeSH
- erytrocyty účinky záření MeSH
- fluorescenční barviva MeSH
- laserová terapie s nízkou intenzitou světla MeSH
- lasery * MeSH
- lidé MeSH
- pyrimidinony MeSH
- sodíko-draslíková ATPasa účinky záření MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fluorescenční barviva MeSH
- merocyanine dye MeSH Prohlížeč
- pyrimidinony MeSH
- sodíko-draslíková ATPasa MeSH
The objective of this study was to investigate the response of Na(+)/K(+)-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 J.cm(-2) and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medium with 2 mM ATP for 30 min. Activity of ATPase was determined colorimetrically by measuring the colored reaction product of liberated inorganic phosphate and malachite green at 640 nm. Contribution of Na(+)/K(+)-ATPase to overall phosphate production was determined using ouabain. A positive effect of green laser light on Na(+)/K(+)-ATPase activity was observed. The dependence of enzymatically liberated inorganic phosphate on light fluence showed a linear correlation (R(2)=0.96, P=0.0005) for all fluences applied (9.5-63.3 J.cm(-2)). On the other hand, MC 540-mediated phototreatment caused a suppression of enzyme activity.
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