Oligonucleotide-based microarray: a new improvement in microarray detection of plant viruses
Language English Country Netherlands Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15927276
DOI
10.1016/j.jviromet.2005.04.009
PII: S0166-0934(05)00134-5
Knihovny.cz E-resources
- MeSH
- Species Specificity MeSH
- Plant Diseases virology MeSH
- Oligonucleotide Probes * MeSH
- Oligonucleotides * chemical synthesis chemistry genetics MeSH
- Plant Viruses classification genetics isolation & purification MeSH
- Oligonucleotide Array Sequence Analysis methods MeSH
- Sensitivity and Specificity MeSH
- Solanum tuberosum virology MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Oligonucleotide Probes * MeSH
- Oligonucleotides * MeSH
Microarrays are one of the new emerging methods in plant virology currently being developed by various laboratories. In this study, a new approach is described on the detection of plant viruses using short synthetic single-stranded oligomers (40 nt) instead of PCR products as capture probes. A microchip detecting potato viruses, PVA, PVS, PVM, PVX, PVY and PLRV, in both single and mixed infections was developed and tested. The chip was also designed to distinguish between the main strains of PVY and PVS. Results of initial tests with PVY(NTN) and PVY(O) strains using several different probes for one virus are presented. Possibilities and advantages of the new oligonucleotide-based microarray approach for plant viral diagnosis are discussed.
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