Nitric oxide-dependent activation of pig oocytes: role of calcium
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15967570
DOI
10.1016/j.mce.2005.05.004
PII: S0303-7207(05)00201-7
Knihovny.cz E-resources
- MeSH
- Egtazic Acid analogs & derivatives pharmacology MeSH
- Heparin pharmacology MeSH
- Macrocyclic Compounds MeSH
- Oocytes drug effects metabolism MeSH
- Oxazoles pharmacology MeSH
- Nitric Oxide metabolism MeSH
- Swine * MeSH
- Procaine pharmacology MeSH
- Ruthenium Red pharmacology MeSH
- Calcium metabolism MeSH
- Verapamil pharmacology MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester MeSH Browser
- Egtazic Acid MeSH
- Heparin MeSH
- Macrocyclic Compounds MeSH
- Oxazoles MeSH
- Nitric Oxide MeSH
- Procaine MeSH
- Ruthenium Red MeSH
- Calcium MeSH
- Verapamil MeSH
- xestospongin A MeSH Browser
Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions with BAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by a NO-donor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil had significantly reduced the activation rate in SNAP-treated oocytes. The blockage of inositol triphosphate receptors had no effect on the activation of oocytes by a NO-donor. On the other hand, the blockers of ryanodine receptors, procaine and ruthenium red, inhibited the activation of oocytes induced by a NO-donor. These data indicate that the activation of pig oocytes by a NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate receptors that play a key role.
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