Nitric oxide-dependent activation of pig oocytes: role of calcium
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15967570
DOI
10.1016/j.mce.2005.05.004
PII: S0303-7207(05)00201-7
Knihovny.cz E-zdroje
- MeSH
- EGTA analogy a deriváty farmakologie MeSH
- heparin farmakologie MeSH
- makrocyklické sloučeniny MeSH
- oocyty účinky léků metabolismus MeSH
- oxazoly farmakologie MeSH
- oxid dusnatý metabolismus MeSH
- prasata * MeSH
- prokain farmakologie MeSH
- rutheniová červeň farmakologie MeSH
- vápník metabolismus MeSH
- verapamil farmakologie MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester MeSH Prohlížeč
- EGTA MeSH
- heparin MeSH
- makrocyklické sloučeniny MeSH
- oxazoly MeSH
- oxid dusnatý MeSH
- prokain MeSH
- rutheniová červeň MeSH
- vápník MeSH
- verapamil MeSH
- xestospongin A MeSH Prohlížeč
Pig oocytes matured in vitro are parthenogenetically activated after treatment with nitric oxide (NO)-donor SNAP. The chelation of intracellular calcium ions with BAPTA-AM suppressed the SNAP-induced activation in a dose-dependent manner. Activation by a NO-donor is dependent on the influx of calcium from extracellular spaces, because the blockage of calcium channels by verapamil had significantly reduced the activation rate in SNAP-treated oocytes. The blockage of inositol triphosphate receptors had no effect on the activation of oocytes by a NO-donor. On the other hand, the blockers of ryanodine receptors, procaine and ruthenium red, inhibited the activation of oocytes induced by a NO-donor. These data indicate that the activation of pig oocytes by a NO-donor is calcium-dependent. The calcium for the activation is mobilized from extracellular and intracellular spaces. For the mobilization of intracellular calcium stores, it is the ryanodine receptors and not the inositol triphosphate receptors that play a key role.
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