Validated HPLC-MS-MS method for determination of azithromycin in human plasma
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, validační studie
- MeSH
- antibakteriální látky krev chemie MeSH
- azithromycin krev MeSH
- časové faktory MeSH
- EDTA krev MeSH
- hmotnostní spektrometrie metody MeSH
- lidé MeSH
- referenční hodnoty MeSH
- reprodukovatelnost výsledků MeSH
- roxithromycin chemie MeSH
- senzitivita a specificita MeSH
- ultrafiltrace MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- validační studie MeSH
- Názvy látek
- antibakteriální látky MeSH
- azithromycin MeSH
- EDTA MeSH
- roxithromycin MeSH
A validated, highly sensitive, and selective HPLC method with MS-MS detection has been developed for quantitative determination of azithromycin (AZI) in human Na2EDTA plasma. Roxithromycin (ROX) was used as internal standard. Human plasma containing AZI and internal standard was ultrafiltered through Centrifree Micropartition devices and the concentration of AZI was determined by isocratic HPLC-MS-MS. Multiple reaction monitoring mode (MRM) was used for MS-MS detection. The calibration plot was linear in the concentration range 2.55-551.43 ng mL(-1). Inter-day and Intra-day precision and accuracy of the proposed method were characterized by R.S.D and percentage deviation, respectively; both were less than 8%. Limit of quantification was 2.55 ng mL(-1). The proposed method was used to determine the pharmacokinetic profile of AZI (250-mg tablets).
Citace poskytuje Crossref.org
