Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu hodnotící studie, časopisecké články
PubMed
16110916
DOI
10.1007/bf02931460
Knihovny.cz E-zdroje
- MeSH
- Aedes růst a vývoj mikrobiologie MeSH
- chloroform MeSH
- ethanol MeSH
- fixativa MeSH
- histocytologické preparační techniky metody MeSH
- kyselina octová MeSH
- larva mikrobiologie MeSH
- oomycety patogenita fyziologie ultrastruktura MeSH
- spory hub ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Názvy látek
- Carnoy's solution MeSH Prohlížeč
- chloroform MeSH
- ethanol MeSH
- fixativa MeSH
- kyselina octová MeSH
We evaluated which of the fixatives and stains most frequently used for observation of insect tissues were the most appropriate for histopathological visualization of entomopathogenic fungal infections with Leptolegnia chapmanii in larvae of Aedes aegypti. The best contrast between the host tissues and the fungal structures was obtained when using a combination of Camoy fixative with Grocott staining contrasted with light green. Masson trichromic stain combined with 10% formaldehyde-phosphate buffer also provided satisfactory results--a good contrast and clearly distinguishable host tissues and fungal structures.
Zobrazit více v PubMed
Cytobios. 1992;69(278-279):179-86 PubMed
Folia Microbiol (Praha). 2003;48(5):611-8 PubMed
J Invertebr Pathol. 1988 May;51(3):259-74 PubMed
J Invertebr Pathol. 1988 Jan;51(1):92-3 PubMed
Rev Iberoam Micol. 1999 Sep;16(3):143-5 PubMed
J Invertebr Pathol. 1988 Jan;51(1):94-6 PubMed
Mycopathologia. 1988 Nov;104(2):67-73 PubMed
