Three-dimensional reconstruction of anomalous chloroplasts in transgenic ipt tobacco
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- alkyltransferasy a aryltransferasy genetika metabolismus MeSH
- chloroplasty genetika metabolismus ultrastruktura MeSH
- cytokininy genetika metabolismus MeSH
- geneticky modifikované rostliny MeSH
- regulace genové exprese u rostlin MeSH
- rostlinné geny genetika MeSH
- tabák cytologie enzymologie genetika metabolismus MeSH
- transgeny genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adenylate isopentenyltransferase MeSH Prohlížeč
- alkyltransferasy a aryltransferasy MeSH
- cytokininy MeSH
Anomalies in the ultrastructure of chloroplasts, from transgenic ipt tobacco, overproducing endogenous cytokinins (CKs) were studied. Detailed analyses of CKs and their metabolites showed that Pssu-ipt tobacco contained enhanced contents of CKs both in leaves and in isolated chloroplasts. The role of CKs in the formation of anomalous structures is suggested. Pssu-ipt chloroplasts frequently formed the distinct peripheral reticulum with a system of caverns that often involved mitochondria and/or peroxisomes. Large crystalloids, which were found in chloroplasts of Pssu-ipt, occupied up to 16% of chloroplast volume. We suggested that the crystalloids were formed by LHC II aggregates. This was supported by analysis of the fluorescence emission spectra at 77 degrees K, chlorophyll a/b ratio, immunogold staining of the structures, and crystallographic unit size analysis.
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Nature. 1994 Feb 17;367(6464):614-21 PubMed
Photosynth Res. 2001;68(2):153-61 PubMed
Plant Physiol. 1949 Jan;24(1):1-15 PubMed
J Biol Chem. 2004 Apr 2;279(14):14049-54 PubMed
Planta. 2003 Jan;216(3):413-21 PubMed
Plant Physiol. 1997 Feb;113(2):313-319 PubMed
Biochim Biophys Acta. 2003 Sep 30;1606(1-3):105-16 PubMed
Plant Physiol. 1996 Sep;112(1):141-148 PubMed
Prog Lipid Res. 1998 Jul-Aug;37(2-3):119-41 PubMed
Planta. 1988 Jan;173(1):104-9 PubMed
Anal Biochem. 1964 Dec;9:431-42 PubMed
Plant J. 1999 Sep;19(6):635-43 PubMed
Curr Genet. 1982 Nov;6(2):129-35 PubMed
Plant Physiol. 1999 Sep;121(1):245-52 PubMed
Biochim Biophys Acta. 1994 Feb 8;1184(1):1-19 PubMed
Plant Physiol. 1997 Jan;113(1):137-147 PubMed
J Chromatogr A. 2002 Mar 15;950(1-2):21-9 PubMed
Eur J Biochem. 2001 Dec;268(23):6020-8 PubMed
J Cell Biol. 1985 Apr;100(4):1139-47 PubMed
Plant Physiol. 2002 Feb;128(2):354-62 PubMed
Photosynth Res. 2003;77(1):1-19 PubMed
FEBS Lett. 2003 Nov 20;554(3):373-80 PubMed
Physiol Plant. 2003 May;118(1):127-137 PubMed
Plant Physiol. 1997 Apr;113(4):1023-31 PubMed
Plant Mol Biol. 1985 May;5(3):155-63 PubMed
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