Three-dimensional reconstruction of anomalous chloroplasts in transgenic ipt tobacco
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Alkyl and Aryl Transferases genetics metabolism MeSH
- Chloroplasts genetics metabolism ultrastructure MeSH
- Cytokinins genetics metabolism MeSH
- Plants, Genetically Modified MeSH
- Gene Expression Regulation, Plant MeSH
- Genes, Plant genetics MeSH
- Nicotiana cytology enzymology genetics metabolism MeSH
- Transgenes genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- adenylate isopentenyltransferase MeSH Browser
- Alkyl and Aryl Transferases MeSH
- Cytokinins MeSH
Anomalies in the ultrastructure of chloroplasts, from transgenic ipt tobacco, overproducing endogenous cytokinins (CKs) were studied. Detailed analyses of CKs and their metabolites showed that Pssu-ipt tobacco contained enhanced contents of CKs both in leaves and in isolated chloroplasts. The role of CKs in the formation of anomalous structures is suggested. Pssu-ipt chloroplasts frequently formed the distinct peripheral reticulum with a system of caverns that often involved mitochondria and/or peroxisomes. Large crystalloids, which were found in chloroplasts of Pssu-ipt, occupied up to 16% of chloroplast volume. We suggested that the crystalloids were formed by LHC II aggregates. This was supported by analysis of the fluorescence emission spectra at 77 degrees K, chlorophyll a/b ratio, immunogold staining of the structures, and crystallographic unit size analysis.
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