A liquid chromatographic-mass spectrometric evidence of dihydrosanguinarine as a first metabolite of sanguinarine transformation in rat
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16280262
DOI
10.1016/j.jchromb.2005.10.030
PII: S1570-0232(05)00777-4
Knihovny.cz E-resources
- MeSH
- Alkaloids pharmacokinetics MeSH
- Benzophenanthridines MeSH
- Biotransformation MeSH
- Phenanthridines metabolism MeSH
- Spectrometry, Mass, Electrospray Ionization methods MeSH
- Isoquinolines MeSH
- Rats MeSH
- Rats, Wistar MeSH
- Reproducibility of Results MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alkaloids MeSH
- Benzophenanthridines MeSH
- dihydrosanguinarine MeSH Browser
- Phenanthridines MeSH
- Isoquinolines MeSH
- sanguinarine MeSH Browser
Adult rats were orally administered with a single dose of sanguinarine (10 mg SA per 1 kg body weight) in 1.0 ml water. In the plasma and the liver, dihydrosanguinarine (DHSA) was identified as a SA metabolite by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC/ESI-MS). Significantly higher levels of DHSA were found in both the plasma and the liver in comparison with those of SA. SA and DHSA were not detected in the urine. The formation of DHSA might be the first step of SA detoxification in the organism and its subsequent elimination in phase II reactions. Benz[c]acridine (BCA), in the literature cited SA metabolite, was found neither in urine nor in plasma and liver.
References provided by Crossref.org
