Process-independent quantitative assessment of residual biological contamination of medical devices reprocessed in washer-disinfectors
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16295663
DOI
10.1007/bf02931572
Knihovny.cz E-resources
- MeSH
- Disinfection methods standards MeSH
- European Union MeSH
- Cross Infection prevention & control MeSH
- Equipment Contamination prevention & control MeSH
- Humans MeSH
- Equipment Reuse MeSH
- Soil analysis MeSH
- Soil Microbiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Soil MeSH
A method based on measuring a soil-induced fluorescence intensity response of 1,8-anilinonaphthalene sulfonate at two fixed wavelengths (460 and 510 nm) was used for determining residual contamination on test soil carriers simulating medical devices after passage through a hospital washer-disinfector. The fluorescence response can be satisfactorily calibrated to soil levels as low as approximately 1 microg/L. Practical tests were performed in two hospitals with washer-disinfectors of 3 types and with several chemical or enzymic cleansers-disinfectants. In combination with the previously developed system of standardized test soil carriers simulating both easily and poorly accessible parts of soiled medical devices, the liver-lactose-oil test soil and an efficient sonication procedure for stripping the residual soil off the carriers, this soil detection method permits the detection of very low contamination levels.
