Recognition of cisplatin-damaged DNA by p53 protein: critical role of the p53 C-terminal domain
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
16300733
DOI
10.1016/j.bbrc.2005.11.038
PII: S0006-291X(05)02536-2
Knihovny.cz E-zdroje
- MeSH
- cisplatina farmakologie MeSH
- DNA metabolismus MeSH
- monoklonální protilátky imunologie MeSH
- nádorový supresorový protein p53 chemie genetika imunologie metabolismus MeSH
- oxidace-redukce MeSH
- poškození DNA účinky léků MeSH
- sekvenční delece genetika MeSH
- substrátová specifita MeSH
- terciární struktura proteinů MeSH
- vazba proteinů MeSH
- vazebná místa MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cisplatina MeSH
- DNA MeSH
- monoklonální protilátky MeSH
- nádorový supresorový protein p53 MeSH
It was shown previously that the p53 protein can recognize DNA modified with antitumor agent cisplatin (cisPt-DNA). Here, we studied p53 binding to the cisPt-DNA using p53 deletion mutants and via modulation of the p53-DNA binding by changes of the protein redox state. Isolated p53 C-terminal domain (CTD) bound to the cisPt-DNA with a significantly higher affinity than to the unmodified DNA. On the other hand, p53 constructs involving the core domain but lacking the C-terminal DNA binding site (CTDBS) exhibited only small binding preference for the cisPt-DNA. Oxidation of cysteine residues within the CD of posttranslationally unmodified full length p53 did not affect its ability to recognize cisPt-DNA. Blocking of the p53 CTDBS by a monoclonal antibody Bp53-10.1 resulted in abolishment of the isolated CTD binding to the cisPt-DNA. Our results demonstrate a crucial role of the basic region of the p53 CTD (aa 363-382) in the cisPt-DNA recognition.
Citace poskytuje Crossref.org
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