HPLC in reversed phase mode: Tool for investigation of kinetics of blackcurrant seed oil lipolysis in supercritical carbon dioxide
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16597516
DOI
10.1016/j.jchromb.2006.03.035
PII: S1570-0232(06)00256-X
Knihovny.cz E-resources
- MeSH
- Diglycerides analysis isolation & purification MeSH
- Hydrolysis MeSH
- Kinetics MeSH
- Linolenic Acids chemistry metabolism MeSH
- Lipolysis * MeSH
- Fatty Acids analysis chemistry MeSH
- Nuclear Magnetic Resonance, Biomolecular MeSH
- Plant Oils chemistry metabolism MeSH
- Carbon Dioxide chemistry MeSH
- Reference Standards MeSH
- Chromatography, Supercritical Fluid methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,2-dilinoleoylglycerol MeSH Browser
- blackcurrant seed oil MeSH Browser
- Diglycerides MeSH
- Linolenic Acids MeSH
- Fatty Acids MeSH
- Plant Oils MeSH
- Carbon Dioxide MeSH
Blackcurrant (Ribes nigrum) seed oil is rich in alpha- and gamma-linolenic acids, the latter in particular being of potential use in medicine. The enzymatic hydrolysis of the oil was carried out in supercritical carbon dioxide using lipase Lipozyme as catalyst and changes in the composition of acylglycerols were recorded. Mono-, di-, and triacylglycerols and free fatty acids were separated by non-aqueous high-performance liquid chromatography in reversed phase mode and detected by UV diode array and 1H NMR detectors. Lipozyme was found to exert low specificity to individual fatty acids in the hydrolysed oil.
References provided by Crossref.org
A review on the effects of supercritical carbon dioxide on enzyme activity
Plant products for pharmacology: application of enzymes in their transformations
