Basidiomycete cryopreservation on perlite: evaluation of a new method
Language English Country Netherlands Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
16600206
DOI
10.1016/j.cryobiol.2006.02.003
PII: S0011-2240(06)00048-4
Knihovny.cz E-resources
- MeSH
- Basidiomycota * growth & development metabolism MeSH
- Nitrogen MeSH
- Cryopreservation methods MeSH
- Laccase metabolism MeSH
- Aluminum Oxide * MeSH
- Silicon Dioxide * MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nitrogen MeSH
- Laccase MeSH
- Aluminum Oxide * MeSH
- Silicon Dioxide * MeSH
- Perlite MeSH Browser
A new cryopreservation method using perlite as a carrier was evaluated on a large set of mycelial cultures of basidiomycetes. The viability and some other characteristics--growth, macro- and micromorphology, and laccase production--of 442 strains were tested after 48-h and then after 3-year storage in liquid nitrogen using a perlite protocol (PP). All (100%) of them survived successfully both 48-h storage and 3-year storage in liquid nitrogen without noticeable growth and morphological changes. Also laccase production was unchanged. The viability and laccase production of a part (250) of these strains were compared with those of the strains subjected to an original agar plug protocol (OP). Using OP, 144 strains (57.6%) out of 250 survived a 3-year storage in liquid nitrogen. The results indicate that the cryopreservation protocol used significantly influences survival of the strains. Markedly better results were achieved using the PP.
References provided by Crossref.org
Cryopreservation of filamentous micromycetes and yeasts using perlite
