Sex-dependent actions of amyloid beta peptides on hippocampal choline carriers of postnatal rats
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- amyloidní beta-protein farmakologie fyziologie MeSH
- cholin metabolismus MeSH
- difenylhexatrien MeSH
- ELISA MeSH
- fluidita membrány MeSH
- fluorescenční barviva MeSH
- fluorescenční polarizace MeSH
- hipokampus účinky léků růst a vývoj metabolismus MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- membránové transportní proteiny metabolismus MeSH
- peptidové fragmenty farmakologie fyziologie MeSH
- potkani Wistar MeSH
- radioligandová zkouška MeSH
- sexuální faktory MeSH
- synaptozomy účinky léků metabolismus MeSH
- techniky in vitro MeSH
- věkové faktory MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- amyloid beta-protein (1-40) MeSH Prohlížeč
- amyloid beta-protein (1-42) MeSH Prohlížeč
- amyloidní beta-protein MeSH
- cholin MeSH
- choline transporter MeSH Prohlížeč
- difenylhexatrien MeSH
- fluorescenční barviva MeSH
- membránové transportní proteiny MeSH
- peptidové fragmenty MeSH
It is suggested that amyloid beta peptides (Abeta) play a role in the pathogenesis of Alzheimer disease but their physiological function is still unknown. However, low pM-nM concentrations mediate a hypofunction of a basal forebrain cholinergic system without marked signs of neurotoxicity. In this study, we compared in vitro effects of soluble nonaggregated human Abeta 1-40 and 1-42 either on synaptosomal hemicholinium-3 sensitive choline carriers or on membrane fluidity in hippocampi of male and female Wistar rats aged 7 and 14 days or 2-3 months. The results indicate age- and sex-dependent effects mediated by peptides at nM concentrations but no significant differences between both fragments. Namely, opposite actions were observed in 14-day (the increase in the choline uptake and membrane fluidity) when compared to 7-day old and adult males (the mild drops). Lineweaver-Burk plot analysis revealed that the enhancement of the high-affinity choline transport in 14-day old males occurs via alterations in K (M )and the change was accompanied by a mild increase in the specific binding of [3H]hemicholinium-3. On the other hand, no age-dependent differences were found in females. Rat Abeta 1-40 mediated similar effects on 14-day old rats as the corresponding human fragment. Moreover, higher levels of soluble peptides were detected in immature when compared to mature male brains by means of competitive ELISA. Our study indicates that Abeta could play a role in postnatal sexual differentiation of hippocampal cholinergic system.
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Protein τ-mediated effects on rat hippocampal choline transporters CHT1 and τ-amyloid β interactions
