Directional motion of foreign plasmid DNA to nuclear HP1 foci
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biologický transport genetika fyziologie MeSH
- buněčné jádro fyziologie MeSH
- chromozomální proteiny, nehistonové genetika fyziologie MeSH
- DNA genetika fyziologie MeSH
- heterochromatin MeSH
- homolog proteinu s chromoboxem 5 MeSH
- lidé MeSH
- mikroskopie MeSH
- nádorové buněčné linie MeSH
- plazmidy genetika fyziologie MeSH
- transfekce MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chromozomální proteiny, nehistonové MeSH
- DNA MeSH
- heterochromatin MeSH
- homolog proteinu s chromoboxem 5 MeSH
Movement of labelled plasmid DNA relative to heterochromatin foci in nuclei, visualized with HP1-GFP, was studied using live-cell imaging and object tracking. In addition to Brownian motion of plasmid DNA we found a pronounced, non-random movement of plasmid DNA towards the nearest HP1 focus, while time-lapse microscopy showed that HP1 foci are relatively immobile and positionally stable. The movement of plasmid DNA was much faster than that of the HP1 foci. Contact of transgene DNA with an HP1 focus usually resulted in cessation of the directional motion. Moreover, the motion of plasmid DNA inside the heterochromatin compartment was more restricted (limited to 0.25 microm) than when the plasmid DNA was outside heterochromatin (R = 0.7 microm). Three days after transfection most of the foreign labelled DNA colocalized with centromeric heterochromatin.
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