Limited genetic variability in Megasphaera elsdenii strains
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17007430
DOI
10.1007/bf02931818
Knihovny.cz E-resources
- MeSH
- Rumen microbiology MeSH
- DNA, Bacterial genetics MeSH
- DNA Fingerprinting MeSH
- Phenotype MeSH
- Phylogeny MeSH
- Genetic Variation * MeSH
- Genotype MeSH
- Megasphaera classification genetics MeSH
- Microbial Sensitivity Tests methods MeSH
- Molecular Sequence Data MeSH
- Sheep MeSH
- RNA, Ribosomal, 16S classification MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial MeSH
- RNA, Ribosomal, 16S MeSH
Levels of phenotypic and genotypic diversity among seven Megasphaera elsdenii strains recovered from rumen contents of cattle, sheep and lambs were determined by a combination of antibiotic-resistance analysis and PCR fingerprint techniques targeted both to the ribosomal RNA operon (ARDRA, RISA) and the whole genome (ERIC-PCR, RAPD-PCR). Despite exhibiting different antibiotic resistance profiles, the tested strains represent genetically nearly identical isolates. Close genetic relatedness was found among M. elsdenii isolates that originated from vastly different habitats worldwide, as revealed by the comparison of 16S rDNA sequences.
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