Lactate dehydrogenase gene variability among predominant lactate utilizing ruminal bacteria
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- bachor mikrobiologie MeSH
- bakteriální proteiny genetika MeSH
- bodová mutace MeSH
- DNA bakterií chemie genetika MeSH
- fylogeneze MeSH
- genetická variace * MeSH
- kyselina mléčná metabolismus MeSH
- L-laktátdehydrogenasa genetika MeSH
- Megasphaera enzymologie genetika izolace a purifikace metabolismus MeSH
- molekulární sekvence - údaje MeSH
- sekvenční analýza DNA MeSH
- sekvenční homologie MeSH
- Selenomonas enzymologie genetika izolace a purifikace metabolismus MeSH
- shluková analýza MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- kyselina mléčná MeSH
- L-laktátdehydrogenasa MeSH
The inter- and intraspecies variability of lactate dehydrogenase (ldh) gene was determined among the predominant ruminal lactate utilizing bacteria. Nearly complete nucleotide sequences of ldh gene, encoding NAD-dependent lactate dehydrogenase of three Megasphaera elsdenii and six Selenomonas ruminantium strains, were obtained and compared. Phylogenetic analyses revealed a limited variability between the ldh sequences studied. The majority of differences observed were silent mutations at the 3rd position of codons. Surprisingly, the intraspecies diversity of the ldh gene among S. ruminantium isolates was higher than the interspecies level between S. ruminantium and M. elsdenii, which strongly suggests the possibility of acquisition of this gene by horizontal gene transfer.
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GENBANK
GQ365641, GQ365642, GQ365643, GQ365644, GQ365645, GQ365646, GQ365647, GQ365648
