Lactate dehydrogenase gene variability among predominant lactate utilizing ruminal bacteria
Language English Country United States Media print-electronic
Document type Journal Article
- MeSH
- Rumen microbiology MeSH
- Bacterial Proteins genetics MeSH
- Point Mutation MeSH
- DNA, Bacterial chemistry genetics MeSH
- Phylogeny MeSH
- Genetic Variation * MeSH
- Lactic Acid metabolism MeSH
- L-Lactate Dehydrogenase genetics MeSH
- Megasphaera enzymology genetics isolation & purification metabolism MeSH
- Molecular Sequence Data MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology MeSH
- Selenomonas enzymology genetics isolation & purification metabolism MeSH
- Cluster Analysis MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- Lactic Acid MeSH
- L-Lactate Dehydrogenase MeSH
The inter- and intraspecies variability of lactate dehydrogenase (ldh) gene was determined among the predominant ruminal lactate utilizing bacteria. Nearly complete nucleotide sequences of ldh gene, encoding NAD-dependent lactate dehydrogenase of three Megasphaera elsdenii and six Selenomonas ruminantium strains, were obtained and compared. Phylogenetic analyses revealed a limited variability between the ldh sequences studied. The majority of differences observed were silent mutations at the 3rd position of codons. Surprisingly, the intraspecies diversity of the ldh gene among S. ruminantium isolates was higher than the interspecies level between S. ruminantium and M. elsdenii, which strongly suggests the possibility of acquisition of this gene by horizontal gene transfer.
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GENBANK
GQ365641, GQ365642, GQ365643, GQ365644, GQ365645, GQ365646, GQ365647, GQ365648
