Separation, characterization and identification of boar seminal plasma proteins
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17110178
DOI
10.1016/j.jchromb.2006.10.054
PII: S1570-0232(06)00890-7
Knihovny.cz E-resources
- MeSH
- Chromatography, Affinity MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Chromatography, Gel MeSH
- Sperm Capacitation MeSH
- Swine MeSH
- Seminal Plasma Proteins analysis isolation & purification metabolism MeSH
- Reproducibility of Results MeSH
- Protein Binding MeSH
- Chromatography, High Pressure Liquid MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Seminal Plasma Proteins MeSH
- spermadhesin MeSH Browser
Methods used for the isolation, separation and characterization of boar seminal plasma proteins are discussed, as well as techniques applied to study their binding properties. Attention is paid to interactions of these proteins with different types of saccharides and glycoconjugates, with membrane phospholipids, and to interactions between proteins. Boar seminal plasma contains different types of proteins: spermadhesins of the AQN and AWN families; DQH and PSP proteins belong to the most abundant. Some of these proteins are bound to the sperm surface during ejaculation and thus protein-coating layers of sperm are formed. Sperms coated with proteins participate in different types of interactions occurring in the course of the reproduction process, e.g. formation of the oviductal sperm reservoir, sperm capacitation, oocyte recognition and sperm binding to the oocyte.
References provided by Crossref.org
