High-performance liquid chromatography with nuclear magnetic resonance detection--A method for quantification of alpha- and gamma-linolenic acids in their mixtures with free fatty acids
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17157864
DOI
10.1016/j.chroma.2006.11.041
PII: S0021-9673(06)02171-6
Knihovny.cz E-resources
- MeSH
- alpha-Linolenic Acid analysis MeSH
- gamma-Linolenic Acid analysis MeSH
- Fatty Acids, Nonesterified analysis MeSH
- Magnetic Resonance Spectroscopy methods MeSH
- Plant Oils chemistry MeSH
- Ribes chemistry MeSH
- Seeds chemistry MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- alpha-Linolenic Acid MeSH
- gamma-Linolenic Acid MeSH
- Fatty Acids, Nonesterified MeSH
- Plant Oils MeSH
While many naturally occurring mixtures of free fatty acids are conveniently analyzed by hyphenated technique of LC-NMR, a complete separation of alpha- and gamma-linolenic acids for their quantitative determination appears impossible at least by the methods of reversed phase HPLC. However, they can be differentiated and quantified from 1H NMR spectra measured in the course of isocratic acetonitrile-chloroform (90:10, with C8 and C18 columns in series) LC-NMR analysis without the need for any derivatization.
References provided by Crossref.org
LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts
A review on the effects of supercritical carbon dioxide on enzyme activity
Plant products for pharmacology: application of enzymes in their transformations
