Capillary isoelectric focusing of native and inactivated microorganisms
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17328903
DOI
10.1016/j.chroma.2007.02.026
PII: S0021-9673(07)00285-3
Knihovny.cz E-zdroje
- MeSH
- Bacteria účinky léků MeSH
- bakteriofág phi X 174 účinky léků MeSH
- Candida albicans účinky léků MeSH
- elektroforéza kapilární metody MeSH
- Escherichia coli účinky léků MeSH
- isoelektrická fokusace metody MeSH
- izoelektrický bod MeSH
- mikrobiální viabilita MeSH
- pilotní projekty MeSH
- povrchově aktivní látky farmakologie MeSH
- Staphylococcus epidermidis účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- povrchově aktivní látky MeSH
The research of microorganisms includes the development of methods for the inactivation of viruses and other microbes. It also means to efficiently eliminate the infectivity of microorganisms without damage of their integrity and structure. According to the results of the last 5 years the capillary electromigration techniques appear to be very perspective for the comparison of the methods applicable for inactivation in the diagnostics and study of the pathogens. In this paper we suggest the capillary isoelectric focusing of the model microorganisms, Escherichia coli, Staphylococcus epidermidis, Candida albicans and bacteriophage PhiX 174, native or inactivated by different procedures. UV detection and fluorometric detection for the dynamically modified microbes by pyrenebutanoate on the basis of the non-ionogenic tenside were used here. Isoelectric points of native and/or dynamically modified microorganisms and other properties were compared with those obtained after microorganisms inactivation. The segmental injection of the sample pulse enabled the reproducible and efficient capillary isoelectric focusing in different pH gradients. The low-molecular-weight pI markers were used for tracing of the pH gradient.
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