Detekce volných lehkých retezců ("free light chains")--nová metoda diagnostiky hematologickyćh onemocneí
[Detection of free light chains--a new method of diagnostics of haematological diseases]
Language Czech Country Czech Republic Media print
Document type English Abstract, Journal Article
PubMed
17373113
- MeSH
- Biomarkers blood MeSH
- Adult MeSH
- Blood Protein Electrophoresis MeSH
- Immunoelectrophoresis MeSH
- Immunoglobulin Light Chains blood MeSH
- Middle Aged MeSH
- Humans MeSH
- Paraproteinemias diagnosis MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Names of Substances
- Biomarkers MeSH
- Immunoglobulin Light Chains MeSH
BACKGROUND: To diagnose monoclonal gamopathy, one of the most frequent haematological diseases, we use immunochemical assays, which are based on the detection of paraprotein in serum and/or urine. METHODS AND RESULTS: The most common laboratory assays we use are SPE (serum protein electrophoresis) and IFE (immunofixation electrophoresis). New method represents the detection of free light chain (FLC) in serum. In our study we compared those three methods (SPE, IFE and FLC) from the point of sensitivity of paraprotein detection. For FLC detection was used Freelite system analyzer (Immunotech Beckman Coulter). We examined 51 patients with diagnosis of multiple myeloma, nonHodgkin's lymphoma, primary amyloidosis and monoclonal gammopathy of undetermined significance. CONCLUSIONS: Detection of FLC is a valuable method which sometimes could specify diagnosis of MG and make the treatment more accurate.
