20-Hydroxyecdysone prevents oxidative stress damage in adult Pyrrhocoris apterus
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17570141
DOI
10.1002/arch.20182
Knihovny.cz E-resources
- MeSH
- Biomarkers MeSH
- Ecdysterone metabolism pharmacology MeSH
- gamma-Glutamyltransferase metabolism MeSH
- Glutathione metabolism MeSH
- Hemolymph chemistry MeSH
- Herbicides pharmacology MeSH
- Heteroptera drug effects metabolism MeSH
- Insect Proteins analysis MeSH
- Oxidative Stress drug effects MeSH
- Paraquat pharmacology MeSH
- Sex Characteristics MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Ecdysterone MeSH
- gamma-Glutamyltransferase MeSH
- Glutathione MeSH
- Herbicides MeSH
- Insect Proteins MeSH
- Paraquat MeSH
Injections of 38 pmol paraquat (1,1'-dimethyl-4,4'-bypyridilium) into adult Pyrrhocoris apterus (average body weight 29.6 mg in males and 36.9 mg in females) caused a significant elevation of lipid peroxidation and protein carbonylation and a decline of membrane fluidity in the microsomal brain fraction. Another manifestation of oxidative stress was a depletion of the reduced glutathione pool and reduction of the gamma-glutamyl transpeptidase activity in the brain extracts. The damaging action of paraquat on the brain was counteracted by simultaneous injection of 1 pmol 20-hydroxyecdysone (20E). 20E restrained lipid peroxidation and the formation of protein carbonyls, ameliorated changes in microsomal membrane fluidity, enhanced the level of reduced glutathione, and upregulated the activity of gamma-glutamyl transpeptidase. At the organismic level, 20E curtailed three detrimental effects caused by paraquat injection: the disappearance of a blood protein, the suppression of fecundity and egg hatchability, and the shortening of adult life span. The data showed that 20E provided a systemic antioxidant protection but the significance of endogenous ecdysteroids in the management of oxidative stress remains to be shown.
References provided by Crossref.org
Hormonal Regulation of Response to Oxidative Stress in Insects-An Update
