Ordered expression of virulence genes in Salmonella enterica serovar typhimurium
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17575908
DOI
10.1007/bf02932148
Knihovny.cz E-zdroje
- MeSH
- bakteriální geny genetika MeSH
- bakteriální proteiny metabolismus MeSH
- časové faktory MeSH
- glukosa MeSH
- kultivační média MeSH
- promotorové oblasti (genetika) genetika MeSH
- regulace genové exprese u bakterií * MeSH
- Salmonella typhimurium genetika růst a vývoj patogenita MeSH
- salmonelóza mikrobiologie MeSH
- trans-aktivátory metabolismus MeSH
- virulence genetika MeSH
- žlučové kyseliny a soli MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- glukosa MeSH
- HilA protein, Salmonella MeSH Prohlížeč
- kultivační média MeSH
- trans-aktivátory MeSH
- žlučové kyseliny a soli MeSH
Using transcriptional promoter fusions, we investigated the expression of selected SPI-1 and SPI-2 genes of Salmonella enterica serovar Typhimurium (S. Typhimurium). Promoters of genes related to the invasion of the epithelial cell (hilA, hilC, hilD, invF, sicA, sopA, sopB and sopE2) were active in Luria-Bertani (LB) medium and LB with butyrate but were suppressed by bile salts and in glucose minimal (M9) medium. Genes related to S. Typhimurium intracellular survival (phoP, ssrA, ssaB, ssaG, sifA, sifB and pipB) were characterized by their expression in stationary phase in LB and M9 medium. Activity of phoP and ssrA promoters indicated that these might be expressed inside the gut. SPI-1 genes were expressed on the transition to stationary phase while SPI-2 genes were expressed in stationary phase. Among SPI-1 genes, those with regulatory functions preceded in expression the effector genes and sop genes were expressed in the order of sopA, sopB and sopE2, showing hierarchy in the expression of S. Typhimurium virulence genes.
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