Murine homodimeric adhesion/growth-regulatory galectins-1, -2 and -7: comparative profiling of gene/ promoter sequences by database mining, of expression by RT-PCR/immunohistochemistry and of contact sites for carbohydrate ligands by computational chemistry
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
17706016
PII: file/6126/fb2007a0017.pdf
Knihovny.cz E-resources
- MeSH
- Databases, Nucleic Acid * MeSH
- Dimerization MeSH
- Galectin 1 chemistry genetics metabolism MeSH
- Galectin 2 chemistry genetics metabolism MeSH
- Galectins chemistry genetics metabolism MeSH
- Immunohistochemistry MeSH
- Carbohydrate Conformation MeSH
- Ligands MeSH
- RNA, Messenger genetics metabolism MeSH
- Molecular Sequence Data MeSH
- Mice MeSH
- Organ Specificity MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Promoter Regions, Genetic genetics MeSH
- Gene Expression Regulation MeSH
- Carbohydrates chemistry MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Gene Expression Profiling * MeSH
- Transcription Factors metabolism MeSH
- Binding Sites MeSH
- Computational Biology * MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Galectin 1 MeSH
- Galectin 2 MeSH
- Galectins MeSH
- Lgals7 protein, mouse MeSH Browser
- Ligands MeSH
- RNA, Messenger MeSH
- Carbohydrates MeSH
- Transcription Factors MeSH
Following the detection of individual members of the family of galectins it is an obvious challenge to define the extent of functional overlap/divergence among these proteins. As a step to address this issue a comparative profiling has been started in the mouse as a model organism, combining sequence analysis, expression patterns and structural features in the cases of the homodimeric galectins-1, -2 and -7. Close relationship was apparent at the level of global gene organization. Scrutiny of the proximal promoter regions for putative transcription-factor-binding sites by two search algorithms uncovered qualitative and quantitative differences with potential to influence the combinatorial functionality of regulatory sequences. RT-PCR mapping with samples from an array of 17 organs revealed significant differences, separating rather ubiquitous gene expression of galectin-1 from the more restricted individual patterns of galectins-2 and -7. Using specific antisera obtained by affinity depletion including stringent controls to ascertain lack of cross-reactivity these results were corroborated at the level of galectin localization in fixed tissue sections. Nuclear presence was seen in the case of galectin-1. In addition to nonidentical expression profiles the mapping of the carbohydrate recognition domains of galectins-1 and -7 by homology modelling and docking of naturally occurring complex tetra- and pentasaccharides disclosed a series of sequence deviations which may underlie disparate affinities for cell surface glycans/glycomimetic peptides. In view of applicability the presented data can serve as useful reference to delineate changes with respect to disease and in genetically engineered models. To enable more general conclusions on the galectin network it is warranted to further pursue this combined approach within this lectin family.
