Nitric oxide production in mouse and rat macrophages: a rapid and efficient assay for screening of drugs immunostimulatory effects in human cells
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17723311
DOI
10.1016/j.niox.2007.06.006
PII: S1089-8603(07)00066-3
Knihovny.cz E-zdroje
- MeSH
- adenin farmakologie MeSH
- adjuvancia imunologická farmakologie MeSH
- dusitany metabolismus MeSH
- interferon gama metabolismus farmakologie MeSH
- interleukin-10 metabolismus MeSH
- krysa rodu Rattus MeSH
- kultivované buňky MeSH
- leukocyty mononukleární cytologie účinky léků metabolismus MeSH
- lidé MeSH
- lipopolysacharidy farmakologie MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- organofosfonáty farmakologie MeSH
- oxid dusnatý metabolismus MeSH
- peritoneální makrofágy cytologie účinky léků metabolismus MeSH
- potkani inbrední LEW MeSH
- preklinické hodnocení léčiv metody MeSH
- TNF-alfa metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adenin MeSH
- adjuvancia imunologická MeSH
- dusitany MeSH
- interferon gama MeSH
- interleukin-10 MeSH
- lipopolysaccharide, E coli O55-B5 MeSH Prohlížeč
- lipopolysacharidy MeSH
- N(6)-cyclopropyl-9-(2-phosphonylmethoxyethyl)-2,6-diaminopurine MeSH Prohlížeč
- organofosfonáty MeSH
- oxid dusnatý MeSH
- TNF-alfa MeSH
Activation of inducible nitric oxide (NO) synthase (iNOS) and resulting high-output NO release is known to depend on the action of cytokines. We investigated in vitro production of NO by resident peritoneal macrophages from mice and rats, and secretion of cytokines by these cells as well as by human peripheral blood mononuclear cells (PBMC). The cells were cultured in the presence of a selected group of acyclic nucleoside phosphonates that have previously been shown to possess immunobiological potential. Several of the compounds enhanced production of NO in animal macrophages. This activity was associated with stimulatory effects on secretion of cytokines such as TNF-alpha in all mouse and rat macrophages and human PBMC, and IL-10 in mouse and human cells. Statistically highly significant correlation between the range of NO biosynthesis in rodent cells and extent of cytokine stimulation in human PBMC has been observed. It is suggested that the NO assay may be regarded as an efficient, economical and relatively reliable tool in primary screening for intrinsic immunostimulatory activity of compounds in human cell system, at least from the point of view of cytokine secretion.
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