Multichannel SPR biosensor for detection of endocrine-disrupting compounds
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Atrazine analysis MeSH
- Benzo(a)pyrene analysis MeSH
- Biosensing Techniques instrumentation methods MeSH
- Time Factors MeSH
- Endocrine Disruptors analysis MeSH
- Phenols analysis MeSH
- Immunoassay methods MeSH
- 2,4-Dichlorophenoxyacetic Acid analysis MeSH
- Surface Plasmon Resonance instrumentation methods MeSH
- Sensitivity and Specificity MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 4-nonylphenol MeSH Browser
- Atrazine MeSH
- Benzo(a)pyrene MeSH
- Endocrine Disruptors MeSH
- Phenols MeSH
- 2,4-Dichlorophenoxyacetic Acid MeSH
A surface plasmon resonance (SPR) biosensor for simultaneous detection of multiple organic pollutants exhibiting endocrine-disrupting activity, namely atrazine, benzo[a]pyrene, 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-nonylphenol, is reported. The biosensor utilizes a multichannel SPR sensor based on wavelength modulation of SPR and wavelength division multiplexing (WDM) of sensing channels, antibodies as biorecognition element and a competitive immunoassay detection format. An analysis time of 45 min (including 30-min incubation of the sample with antibodies) and limits of detection as low as 0.05, 0.07, 0.16 and 0.26 ng mL(-1) are demonstrated for benzo[a]pyrene, atrazine, 2,4-D and 4-nonylphenol, respectively. The biosensor is also shown to be regenerable and suitable for repeated use.
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