Phenotypic and genotypic analysis of Borrelia spp. isolated from Ixodes ricinus ticks by using electrophoretic chips and real-time polymerase chain reaction
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18062179
DOI
10.1007/bf02932085
Knihovny.cz E-resources
- MeSH
- Antigens, Surface chemistry genetics MeSH
- Bacterial Vaccines chemistry genetics MeSH
- Borrelia burgdorferi Group genetics isolation & purification MeSH
- DNA, Bacterial chemistry genetics MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- Ixodes microbiology MeSH
- Lipoproteins chemistry genetics MeSH
- Microfluidic Analytical Techniques MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- Bacterial Outer Membrane Proteins chemistry genetics MeSH
- Rec A Recombinases chemistry genetics MeSH
- RNA, Ribosomal, 16S chemistry genetics MeSH
- Base Sequence MeSH
- Sequence Alignment MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, Surface MeSH
- Bacterial Vaccines MeSH
- DNA, Bacterial MeSH
- Lipoproteins MeSH
- OspA protein MeSH Browser
- Bacterial Outer Membrane Proteins MeSH
- Rec A Recombinases MeSH
- RNA, Ribosomal, 16S MeSH
The genotype of Borrelia burgdorferi sensu lato was detected in 371 out of 1244 ticks. Borrelia determination was based on partial sequencing of the 16S rRNA gene and real-time polymerase chain reactions for identification and quantitation of ospA and recA genes. Different Borrelia spp. were identified; B. garinii in 40% ticks followed by B. afzelii (36.3%), B. burgdorferi sensu stricto (12.9%), B. valaisiana (3.5%), B. lusitaniae (0.8%), B. bissettii (0.5%) and B. miyamotoi-like (0.5%). Cultivation of 30 borrelia strains in BSK-H medium, among them B. valaisiana, B. bissettii-like and B. miyamotoi-like strains was unique in Czechia. Calibrated microfluidic-based quantification showed differences in the concentration of the nucleic acids and molar mass of the outer surface proteins of different Borrelia spp. with standard sensitivity and specificity and was helpful for their identification. The outer surface protein OspA was absent in B. miyamotoi-like and the OspB protein in B. valaisiana, B. lusitaniae and in three subtypes of B. garinii.
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GENBANK
DQ005955, DQ064600, DQ064602, DQ064603, DQ650327, EF364112, EF364113, EF364114, EF364115
