Label-free piezoelectric immunosensor for rapid assay of Escherichia coli
Language English Country Great Britain, England Media print
Document type Evaluation Study, Journal Article
PubMed
18080881
DOI
10.1080/15321810701735120
PII: 788584858
Knihovny.cz E-resources
- MeSH
- Biosensing Techniques methods MeSH
- Electrodes MeSH
- Escherichia coli immunology isolation & purification MeSH
- Quartz chemistry MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Antibodies, Bacterial chemistry immunology MeSH
- Gold chemistry MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- Quartz MeSH
- Antibodies, Bacterial MeSH
- Gold MeSH
A piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of the piezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 10(6)-10(9) CFU/mL; signal of the negative control was not statistically relevant in the selected range. Samples could be analyzed in four minutes and one measuring cycle including regeneration was completed within ten minutes. Repeatability of the developed method is discussed; the signal obtained from three different biosensors was 12.9+/-0.4 Hz for the sample containing 10(8) CFU/mL.
References provided by Crossref.org
Amperometric Biosensors for Real Time Assays of Organophosphates
