Piezoelectric biosensor was used for diagnosis of infection by Francisellatularensis subsp. holarctica in European brown hares. Two kinds of experiments wereperformed in this study. First, sera from experimentally infected European brown hares(Lepus europaeus) were assayed by piezoelectric biosensor and the seventh day postinfection was found as the first one when statistically significant diagnosis of tularemia waspossible; all other sera collected from hares later than on day 7 following the infection werefound tularemia positive. Typing to classify the field strain of F. tularensis used for theexperimental infection was confirmed by proteome study. Second, sera from 35 Europeanbrown hare specimens sampled at hunting grounds and tested as tularemia positive by slowagglutination allowed diagnosis of tularemia by the piezoelectric biosensor. All these sera ofnaturally infected hares were found as tularemia positive, too. Efficacy of the piezoelectricbiosensor for the serological diagnosis of tularemia is discussed.

Center of Biological Defense Těchonín Czech Republic

Department of Infectious Diseases and Epizootiology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic

Department of Veterinary Ecology and Environmental Protection University of Veterinary and Pharmaceutical Sciences Brno Czech Republic

Institute of Molecular Pathology University of Defence Hradec Králové Czech Republic

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Dennis D.T., Inglesby T.V., Henderson D.A., Bartlett J.G., Ascher M.S., Eitzen E., Fine A.D., Friedlander A.M., Hauer J., Layton M., Lilibridge S.R., McDade J.E., Osterholm M.T., O'Toole T., Parker G., Perl T.M., Russell P.K., Tonat K. Tularemia as a biological weapon – Medical and public health management. J. Am. Med. Assoc. 2001;285:2763–2773. PubMed

Pikula J., Treml F., Beklova M., Holesovska Z., Pikulova J. Geographic information systems in epidemiology – ecology of common vole and distribution of natural foci of tularaemia. Acta Vet. Brno. 2002;71:379–387.

Pikula J., Treml F., Berklova M., Holesovska Z., Pikulova J. Ecological conditions of natural foci of tularaemia in the Czech Republic. Eur. J. Epidemiol. 2003;18:1091–1095. PubMed

Pikula J., Beklova M., Holesovska Z., Treml F. Ecology of european brown hare and distribution of natural foci of tularaemia in the Czech Republic. Acta Vet. Brno. 2004;73:267–273.

Hubalek Z., Sixl W., Halouzka J. Francisella tularensis in Dermacentor reticulatus ticks from the Czech Republic and Austria. Wien. Klin. Wochenschr. 1998;110:909–910. PubMed

Hubalek Z., Treml F., Juricova F., Hunady M., Halouzka J., Janik V., Bill D. Serological survey of the wild boar (Sus scrofa) for tularaemia and brucellosis in South Moravia, Czech Republic. Vet. Med. – Czech. 2002;47:60–66.

Siret V., Barataud D., Prat M., Vaillant V., Ansart S., LeCoustumier A., Vaissaire J., Raffi F., Garre M., Capek I. An outbreak of airborne tularemia in France, August 2004. Euro. Surveill. 2006;11:58–60. PubMed

Forsman M., Sandstrom G., Jaurin B. Identification of Francisella species and discrimination of type A and type B strains of F. tularensis by 16S rRNA analysis. Appl. Environ. Microbiol. 1990;56:949–955. PubMed PMC

Svensson K., Larsson P., Johansson D., Bystrom M., Forsman M., Johansson A. Evolution of subspecies of Francisella tularensis. J. Bacteriol. 2005;187:3903–3908. PubMed PMC

Gurycova D. First isolation of Francisella tularensis subsp. tularensis in Europe. Eur. J. Epidemiol. 1998;46:797–802. PubMed

Olsufjev N.G., Emelyanova O.S., Dunaeva T.N. Comparative study of strains of B. tularense in the Old and New World and their taxonomy. J. Hyg. Epidemiol. Microbiol. Immunol. 1959;3:138–149. PubMed

Sandstrom G., Sjostedt A., Forsman M., Pavlovich N.V., Mishankin B.N. Characterization and classification of strains of Francisella tularensis isolated in the central asian focus of the Soviet Union and in Japan. J. Clin. Microbiol. 1992;30:172–175. PubMed PMC

Olsufjev N.G., Meshcheryakova I.S. Subspecific taxonomy of Francisella tularensis McCoy and Chapin 1912. Int. J. Syst. Bacteriol. 1983;33:872–874.

Zhang F., Liu W., Chu M.C., He J., Duan Q., Wu X.M., Zhang P.H., Zhao Q.M., Yang H., Xin Z.T., Cao W.C. Francisella tularensis in rodents, China. Emerg. Infect. Dis. 2006;12:994–996. PubMed PMC

Splettstoesser W.D., Tomasu H., Al Dahouk S., Neubauer H., Schuff-Werner P. Diagnostic procedures in tularaemia with special focus on molecular and immunological techniques. J. Vet. Med. B. 2005;52:249–261. PubMed

Pohanka M., Skladal P., Kroca M. Biosensors for biological warfare agent detection. Def. Sci. J. 2007;57:185–193.

Pohanka M., Skládal P. Serological diagnosis of tularemia in mice using the amperometric immunosensor. Electroanalysis. in press.

Pohanka M., Jun D., Kuca K. Mycotoxin assays using biosensor technology: a review. Drug. Chem. Toxicol. 2007;30:253–261. PubMed

Pohanka M., Skladal P., Pavlis O. A label free piezoelectric immunosensor for rapid assay of Escherichia coli. J. Immunoass. Immunoch. 2008;29 in press. PubMed

Vitecek J., Petrlova J., Adam V., Havel L., Kramer K.J., Babula P., Kizek R. A fluorimetric sensor for detection of one living cell. Sensors. 2007;7:222–238.

Skladal P. Piezoelectric quartz crystal sensors applied for bioanalytical assays and characterization of affinity interactions. J. Braz. Chem. Soc. 2003;14:491–502.

Kreutz C., Lorgen J., Graewe B., Bargon J., Yoshida M., Fresco Z.M., Frechet J.M.J. High frequency quartz micro balances: a promising path to enhanced sensitivity of gravimetric sensors. Sensors. 2006;6:335–340.

Pohanka M., Skladal P. Piezoelectric immunosensor for Francisella tularensis detection using immunoglobulin M in a limiting dilution. Anal. Lett. 2005;38:411–422.

Pohanka M., Skladal P. Piezoelectric immunosensor for the direct and rapid detection of Francisella tularensis. Folia Microbiol. 2007;52:325–330. PubMed

Pohanka M., Pavlis O., Skladal P. Diagnosis of tularemia using piezoelectric biosensor technology. Talanta. 2007;71:981–985. PubMed

Pohanka M., Pavlis O., Skladal P. Rapid characterization of monoclonal antibodies using the piezoelectric immunosensor. Sensors. 2007;7:341–353.

Pohanka M. Evaluation of immunoglobulin production during tularaemia infection in BALB/c mouse model. Acta Vet. Brno. in press.

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