Piezoelectric immunosensor for the direct and rapid detection of Francisella tularensis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18062180
DOI
10.1007/bf02932086
Knihovny.cz E-zdroje
- MeSH
- biologické bojové látky MeSH
- biosenzitivní techniky metody MeSH
- Francisella tularensis izolace a purifikace MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- protilátky bakteriální chemie MeSH
- tularemie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biologické bojové látky MeSH
- protilátky bakteriální MeSH
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detect F. tularensis with the limit of detection 10(5) CFU/mL with a typical measuring cycle > 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
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