Piezoelectric immunosensor for the direct and rapid detection of Francisella tularensis
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18062180
DOI
10.1007/bf02932086
Knihovny.cz E-resources
- MeSH
- Biological Warfare Agents MeSH
- Biosensing Techniques methods MeSH
- Francisella tularensis isolation & purification MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Antibodies, Bacterial chemistry MeSH
- Tularemia microbiology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biological Warfare Agents MeSH
- Antibodies, Bacterial MeSH
A novel immunosensing device based on a piezoelectric sensor for direct detection of the biological warfare agent Francisella tularensis was developed. This sensor includes mouse polyclonal antibody immobilized in a layer of protein A covalently linked to the gold electrode of the sensor. The immunosensor is able to detect F. tularensis with the limit of detection 10(5) CFU/mL with a typical measuring cycle > 5 min. The sensor was successfully evaluated for rapid detection of F. tularensis spikes in drinking water and milk; no deterioration of sensitivity in comparison with buffer solutions was observed. The proposed concept of a rapid measurement of microbial agents seems to be promising for evaluation of samples after short pre-cultivation enrichment.
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