Label-free piezoelectric immunosensor for rapid assay of Escherichia coli
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu hodnotící studie, časopisecké články
PubMed
18080881
DOI
10.1080/15321810701735120
PII: 788584858
Knihovny.cz E-zdroje
- MeSH
- biosenzitivní techniky metody MeSH
- elektrody MeSH
- Escherichia coli imunologie izolace a purifikace MeSH
- křemen chemie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- protilátky bakteriální chemie imunologie MeSH
- zlato chemie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- Názvy látek
- křemen MeSH
- protilátky bakteriální MeSH
- zlato MeSH
A piezoelectric sensor with immobilized polyclonal antibody was developed as a label-free assay for the model bacterium, Escherichia coli. The polyclonal antibody was prepared from mice BALB/c and covalently immobilized on the sensing gold electrode of the piezoelectric quartz crystal. The biosensor was able to detect E. coli in the range of 10(6)-10(9) CFU/mL; signal of the negative control was not statistically relevant in the selected range. Samples could be analyzed in four minutes and one measuring cycle including regeneration was completed within ten minutes. Repeatability of the developed method is discussed; the signal obtained from three different biosensors was 12.9+/-0.4 Hz for the sample containing 10(8) CFU/mL.
Citace poskytuje Crossref.org
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