Glucosylglycerate is an osmotic solute and an extracellular metabolite produced by Streptomyces caelestis
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18298040
DOI
10.1007/bf02932103
Knihovny.cz E-resources
- MeSH
- Disaccharides isolation & purification MeSH
- Extracellular Space chemistry metabolism MeSH
- Glycosylation MeSH
- Culture Media chemistry MeSH
- Glyceric Acids agonists chemistry isolation & purification metabolism MeSH
- Magnetic Resonance Spectroscopy MeSH
- Osmolar Concentration MeSH
- Osmosis MeSH
- Streptomyces chemistry metabolism MeSH
- Trehalose chemistry isolation & purification metabolism MeSH
- Water-Electrolyte Balance * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Disaccharides MeSH
- glyceric acid MeSH Browser
- Culture Media MeSH
- Glyceric Acids MeSH
- Trehalose MeSH
Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.
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