Glucosylglycerate is an osmotic solute and an extracellular metabolite produced by Streptomyces caelestis
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18298040
DOI
10.1007/bf02932103
Knihovny.cz E-zdroje
- MeSH
- disacharidy izolace a purifikace MeSH
- extracelulární prostor chemie metabolismus MeSH
- glykosylace MeSH
- kultivační média chemie MeSH
- kyseliny glycerové agonisté chemie izolace a purifikace metabolismus MeSH
- magnetická rezonanční spektroskopie MeSH
- osmolární koncentrace MeSH
- osmóza MeSH
- Streptomyces chemie metabolismus MeSH
- trehalosa chemie izolace a purifikace metabolismus MeSH
- vodní a elektrolytová rovnováha * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- disacharidy MeSH
- glyceric acid MeSH Prohlížeč
- kultivační média MeSH
- kyseliny glycerové MeSH
- trehalosa MeSH
Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.
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