Decreased susceptibility to antifungals in respiratory-deficient Kluyveromyces lactis mutants
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18298045
DOI
10.1007/bf02932108
Knihovny.cz E-resources
- MeSH
- Amphotericin B pharmacology MeSH
- Antifungal Agents pharmacology MeSH
- Cell Wall chemistry drug effects MeSH
- Glucan Endo-1,3-beta-D-Glucosidase metabolism MeSH
- Drug Resistance, Fungal * MeSH
- Fungal Proteins genetics metabolism MeSH
- Kluyveromyces cytology drug effects genetics physiology MeSH
- Microbial Sensitivity Tests MeSH
- Mitochondria drug effects genetics physiology MeSH
- Multienzyme Complexes metabolism MeSH
- Mutation * MeSH
- Blotting, Northern MeSH
- Peptide Hydrolases metabolism MeSH
- Flow Cytometry MeSH
- Sterols metabolism MeSH
- Electron Transport drug effects genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amphotericin B MeSH
- Antifungal Agents MeSH
- Glucan Endo-1,3-beta-D-Glucosidase MeSH
- Fungal Proteins MeSH
- lyticase MeSH Browser
- Multienzyme Complexes MeSH
- Peptide Hydrolases MeSH
- Sterols MeSH
Decreased susceptibility of K. lactis mutants impaired in the function of cytochrome c, cytochrome c1 and cytochrome-c oxidase to fluconazole, bifonazole and amphotericin B in comparison with the isogenic wild-type strain was observed. Flow cytometry with rhodamine 6G did not show any changes in the accumulation of the dye in the mutant cells compared with the corresponding wild-type strain. Sterol analysis showed similar overall amount of sterols in both wild-type and mutant cells. Taking into account the increased amphotericin B resistance and significantly diminished susceptibility of mutant cells to lyticase digestion, the cell wall structure and/or composition may probably be responsible for the observed changes in the susceptibility of mutants to the antifungal compounds used.
Department of Microbiology and Virology Comenius University in Bratislava 842 15 Bratislava Slovakia
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