Genetic and biological diversity of the Pea seed-borne mosaic virus isolates occurring in Czech Republic
Jazyk angličtina Země Švýcarsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18459836
Knihovny.cz E-zdroje
- MeSH
- fylogeneze MeSH
- genetická variace * MeSH
- hrách setý virologie MeSH
- molekulární sekvence - údaje MeSH
- nemoci rostlin virologie MeSH
- Potyvirus chemie klasifikace genetika izolace a purifikace MeSH
- sekvence aminokyselin MeSH
- sekvenční seřazení MeSH
- virové proteiny chemie genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- virové proteiny MeSH
Eight isolates of the Pea seed-borne mosaic virus (PSbMV) from the Czech Republic were studied regarding their biological and molecular characteristics. Molecular characterization using RT-PCR was done on the 5'(Nter)NIb-CP-UTR3' region amplified using universal CPUP/P9502 primer pair and the newly designed PSB8812/PSB944, and PSB8800/PSB9440 primer pairs, respectively. Sequential and phylogenetic analysis of CP-UTR3' region from all isolates showed that the available Czech and GenBank PSbMV isolates were distributed into 4 clusters in agreement with their diversification and according to their biological characteristics (i.e. pathotype). The molecular data were confirmed by biological testing on different pea cultivars. The Czech isolates were distributed into two pathotypes, the P-1 (7 isolates) and P-4 (1 isolate).
