Genetic and biological diversity of the Pea seed-borne mosaic virus isolates occurring in Czech Republic
Language English Country Switzerland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18459836
Knihovny.cz E-resources
- MeSH
- Phylogeny MeSH
- Genetic Variation * MeSH
- Pisum sativum virology MeSH
- Molecular Sequence Data MeSH
- Plant Diseases virology MeSH
- Potyvirus chemistry classification genetics isolation & purification MeSH
- Amino Acid Sequence MeSH
- Sequence Alignment MeSH
- Viral Proteins chemistry genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Viral Proteins MeSH
Eight isolates of the Pea seed-borne mosaic virus (PSbMV) from the Czech Republic were studied regarding their biological and molecular characteristics. Molecular characterization using RT-PCR was done on the 5'(Nter)NIb-CP-UTR3' region amplified using universal CPUP/P9502 primer pair and the newly designed PSB8812/PSB944, and PSB8800/PSB9440 primer pairs, respectively. Sequential and phylogenetic analysis of CP-UTR3' region from all isolates showed that the available Czech and GenBank PSbMV isolates were distributed into 4 clusters in agreement with their diversification and according to their biological characteristics (i.e. pathotype). The molecular data were confirmed by biological testing on different pea cultivars. The Czech isolates were distributed into two pathotypes, the P-1 (7 isolates) and P-4 (1 isolate).
