Effect of protein degradation on spot M(r) distribution in 2-D gels--a case study of proteolysis during development of Streptomyces coelicolor cultures

. 2008 Jun ; 8 (12) : 2371-5.

Jazyk angličtina Země Německo Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid18563729

Proteolysis, a regulated biological process, is reflected by protein spot molecular weight distribution in 2-D gel electrophoretograms. Here we report studies of Streptomyces cultures as they undergo two different developmental processes involving proteolysis. Systematic changes in protein molecular weight distribution between the control samples and those with high activity of proteases were demonstrated. The observations were supported by a numerical model of degradation and its influence on the M(r) distribution. Simple statistics could be used to distinguish between normal and degradative 2-D gel electrophoretic patterns.

Citace poskytuje Crossref.org

Nejnovějších 20 citací...

Zobrazit více v
Medvik | PubMed

General and molecular microbiology and microbial genetics in the IM CAS

. 2010 Dec ; 37 (12) : 1227-39. [epub] 20101118

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace

Nahrávání dat ...