Effect of protein degradation on spot M(r) distribution in 2-D gels--a case study of proteolysis during development of Streptomyces coelicolor cultures
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Electrophoresis, Gel, Two-Dimensional methods MeSH
- Bacterial Proteins analysis genetics metabolism MeSH
- Time Factors MeSH
- Gels MeSH
- Hydrolysis MeSH
- Calibration MeSH
- Culture Media MeSH
- Molecular Weight MeSH
- Peptide Mapping MeSH
- Reference Standards MeSH
- Streptomyces coelicolor growth & development MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Gels MeSH
- Culture Media MeSH
Proteolysis, a regulated biological process, is reflected by protein spot molecular weight distribution in 2-D gel electrophoretograms. Here we report studies of Streptomyces cultures as they undergo two different developmental processes involving proteolysis. Systematic changes in protein molecular weight distribution between the control samples and those with high activity of proteases were demonstrated. The observations were supported by a numerical model of degradation and its influence on the M(r) distribution. Simple statistics could be used to distinguish between normal and degradative 2-D gel electrophoretic patterns.
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General and molecular microbiology and microbial genetics in the IM CAS
