Time-resolved microspectrofluorometry and fluorescence imaging techniques: study of porphyrin-mediated cellular uptake of oligonucleotides
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
18596340
DOI
10.1196/annals.1430.007
PII: 1130/1/117
Knihovny.cz E-resources
- MeSH
- Oligonucleotides, Antisense chemistry MeSH
- Cell Nucleus metabolism MeSH
- 3T3 Cells MeSH
- Time Factors MeSH
- Fluorescence MeSH
- Microscopy, Fluorescence instrumentation methods MeSH
- Cations MeSH
- Microscopy, Confocal instrumentation methods MeSH
- Drug Delivery Systems MeSH
- Melanoma, Experimental MeSH
- Mice MeSH
- Oligonucleotides chemistry MeSH
- Porphyrins chemistry MeSH
- Sensitivity and Specificity MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Oligonucleotides, Antisense MeSH
- Cations MeSH
- Oligonucleotides MeSH
- Porphyrins MeSH
Time-resolved confocal microspectrofluorometry and fluorescence microscopy imaging were applied to monitor the cellular uptake of fluorescent-labeled oligonucleotides (ONs) delivered by a porphyrin molecule. The fate of porphyrin-ON complexes inside living cells has also been monitored. Due to intrinsic fluorescence of the porphyrin and sensitivity of its characteristics to microenvironment, multicomponent analysis of time-resolved fluorescence provides unique information about stability of the porphyrin-ON complexes, ON interactions with their target sequences, and ON and porphyrin distributions after delivery inside the cells. Time-resolved confocal microspectrofluorometry indeed delivers additional information compared with fluorescence confocal microscopy imaging widely employed to study ON uptake.
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