Photometric microplate assay for estimation of the efficacy of paraoxon-inhibited acetylcholinesterase reactivation
Language English Country Great Britain, England Media print
Document type Journal Article
PubMed
18615283
DOI
10.1080/14756360701811023
PII: 794859466
Knihovny.cz E-resources
- MeSH
- Acetylcholinesterase metabolism MeSH
- Enzyme Activation drug effects MeSH
- Cholinesterase Inhibitors chemistry pharmacology MeSH
- Photometry MeSH
- Paraoxon chemistry pharmacology MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acetylcholinesterase MeSH
- Cholinesterase Inhibitors MeSH
- Paraoxon MeSH
Photometric microplate assay was performed for testing of paraoxon-inhibited acetylcholinesterase (AChE) using three reactivators for reactivation purposes: obidoxime, pralidoxime, and HI-6. 3-D graphs (percent of reactivation vs. concentration of reactivator and vs. time of reactivator effecting) were constructed for each reactivator to compare their efficacy. The best results were obtained using obidoxime where reactivation was near to 80%. Suitability of photometric microplates for following of reactivation procedures is discussed.
References provided by Crossref.org
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