Partial characterization of Enterococcus faecalis bacteriophage F4
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bacteriophages classification genetics physiology MeSH
- Enterococcus faecalis virology MeSH
- Genome, Viral MeSH
- Lysogeny MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction MeSH
- Sequence Analysis, DNA MeSH
- Viral Proteins genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Viral Proteins MeSH
Large Enterococcus faecalis F4 bacteriophage (described earlier) consisting of double-stranded linear DNA of approximately 60 kb was characterized. Library was prepared of its random DNA fragments and selected recombinants were sequenced. Three phage essential genes were characterized: DNA polymerase, replicative DNA helicase and a minor capsid protein, showing only limited homology to other known phage encoded genes. The occurrence of these genes among enterococci was determined by PCR method. Only two out of 40 tested isolates possessed all three genes, another three isolates contained at least one of the genes, demonstrating low frequency F4 lysogens among natural enterococcal isolates.
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GENBANK
EF653454
