Vliv typu růstového faktoru na fenotyp subpopulací a kinetiku CD34+ bunek v periferní krvi a stepech periferních kmenových bunek pacientů s non-hodgkinskými lymfomy indikovaných k autologní transplantaci krvetvorby
[Effect of growth factor on the phenotype of subpopulations and on the kinetics of CD34+ cells in the peripheral blood and in grafts of peripheral stem cells in patients with non-Hodgkin's lymphoma indicated for autologous peripheral blood stem cell transplantation]
Jazyk čeština Země Česko Médium print
Typ dokumentu anglický abstrakt, časopisecké články
PubMed
18724529
- MeSH
- antigeny CD34 analýza MeSH
- dospělí MeSH
- faktor stimulující kolonie granulocytů farmakologie MeSH
- filgrastim MeSH
- imunofenotypizace MeSH
- interleukin-3 farmakologie MeSH
- kmenové buňky imunologie MeSH
- leukaferéza MeSH
- leukocyty imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mobilizace hematopoetických kmenových buněk * MeSH
- nehodgkinský lymfom terapie MeSH
- rekombinantní fúzní proteiny farmakologie MeSH
- rekombinantní proteiny MeSH
- transplantace periferních kmenových buněk * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- antigeny CD34 MeSH
- faktor stimulující kolonie granulocytů MeSH
- filgrastim MeSH
- interleukin-3 MeSH
- leridistim MeSH Prohlížeč
- rekombinantní fúzní proteiny MeSH
- rekombinantní proteiny MeSH
BACKGROUND: Peripheral blood stem cells are the preferred source for transplantation of hematopoiesis in patients with non-Hodgkin's lymphoma. Application of hematopoietic growth factors is a part of the mobilization chemotherapy regimen. Time overlap of the highest leukocyte and CD34+ cell count is required for optimal graft collection. Authors analyzed the effect of two growth factors (leridistim and filgrastim) on the kinetics and phenotype of CD34+ cells in patients with non-Hodgkin's lymphoma indicated for autologous peripheral blood stem cell transplantation. METHODS AND RESULTS: Authors analyzed phenotype of CD34+ cell subpopulations and their kinetics in peripheral blood and leukapheresis products by flow cytometry during mobilization and graft collection. Statistically significant differences in expression of lineage-committed antigens between growth factors were found (CD3, CD5--T-lineage, CD56 NK-lineage, CD20 for B-lineage, p < 0.05), as well as for lineage non-specific antigens (CD38, p < 0.05 and CD54, p < 0.01). The most significant divergence was observed between CD34+CD19+ subpopulations of leridistim and filgrastim stimulated blood and graft (p < 0.001). CONCLUSIONS: Expression of lineage-committed antigens on CD34+ subpopulations between two growth factors was statistically different. Kinetics of CD34+ cells during mobilization regimen with leridistim was not superior to filgrastim concerning the quality of graft.
