High-resolution melting analysis for detection of MYH9 mutations
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18925516
DOI
10.1080/09537100802140013
PII: 904255770
Knihovny.cz E-zdroje
- MeSH
- lidé MeSH
- missense mutace * MeSH
- molekulární motory chemie genetika MeSH
- mutační analýza DNA metody MeSH
- těžké řetězce myosinu chemie genetika MeSH
- tranzitní teplota MeSH
- trombocytopenie krev genetika MeSH
- trombocyty patologie MeSH
- velikost buňky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- molekulární motory MeSH
- MYH9 protein, human MeSH Prohlížeč
- těžké řetězce myosinu MeSH
May-Hegglin anomaly (MHA), Sebastian (SBS), Fechtner (FTNS) and Epstein (EPS) syndromes are rare autosomal dominant disorders with giant platelets and thrombocytopenia. Other manifestations of these disorders are combinations of the presence of granulocyte inclusions and deafness, cataracts and renal failure. Currently, MHA, SBS, FTNS and EPS are considered to be distinct clinical manifestation of a single illness caused by mutations of the MYH9 gene encoding the heavy chain of non-muscle myosin IIA (NMMHC-IIA). As the MYH9 gene has a high number of exons, it takes much time and material to use this method for the detection of MYH9 mutations. Recently, a new method has been introduced for scanning DNA mutations without the need for direct sequencing: high-resolution melting analysis (HRMA). Mutation detection with HRMA relies on the intercalation of the specific dye (LC Green plus) in double-strand DNA and fluorescence monitoring of PCR product melting profiles. In our study, we optimized the conditions and used HRMA for rapid screening of mutations in all MYH9 exons in seven affected individuals from four unrelated families with suspected MYH9 disorders. Samples identified by HRMA as positive for the mutation were analysed by direct sequencing. HRMA saved us over 85% of redundant sequencing.
Citace poskytuje Crossref.org