Biliary 1-hydroxypyrene as a biomarker of exposure to polycyclic aromatic hydrocarbons in fish
Language English Country Sweden Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18987610
PII: NEL290508A01
Knihovny.cz E-resources
- MeSH
- Biomarkers MeSH
- Water Pollutants, Chemical analysis toxicity MeSH
- Cyprinidae physiology MeSH
- Spectrometry, Fluorescence MeSH
- Environmental Monitoring MeSH
- Mutagens metabolism MeSH
- Polycyclic Aromatic Hydrocarbons analysis toxicity MeSH
- Proteins metabolism MeSH
- Pyrenes metabolism MeSH
- Fishes metabolism MeSH
- Fresh Water MeSH
- Chromatography, High Pressure Liquid MeSH
- Bile metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- 1-hydroxypyrene MeSH Browser
- Biomarkers MeSH
- Water Pollutants, Chemical MeSH
- Mutagens MeSH
- Polycyclic Aromatic Hydrocarbons MeSH
- Proteins MeSH
- Pyrenes MeSH
OBJECTIVES: The aim of this study was the assessment of the Svitava and Svratka rivers contamination by polycyclic aromatic hydrocarbons (PAH) using 1-hydroxypyrene (1-OHP) concentrations in fish bile as a biomarker. DESIGN: Levels of 1-OHP were determined by reverse phase HPLC with fluorescence detection. For valid assessment of bile accumulation levels, the 1-OHP concentration was normalized to the biliary protein content. The content of 1-OHP was correlated with the PAH content of river bottom sediments. RESULTS: The highest level of 1-OHP in fish bile (136.1 ng.mg-1 protein) was found at the locality Rajhradice, which is situated downstream of Brno. Also the greatest level of sum of PAHs was found at this locality (17.1 microg.g-1 dry mass). Significant positive correlation (p< or =0.05) between the level of 1-OHP and sum of PAHs in sediment was found only in case of sediments collected in the same month as fish samples (in June). CONCLUSION: Our results document that 1-OHP in fish bile is a suitable biochemical marker for the assessment of aquatic ecosystem contamination by PAHs.
1-Hydroxypyrene--a biochemical marker for PAH pollution assessment of aquatic ecosystem
Can thiol compounds be used as biomarkers of aquatic ecosystem contamination by cadmium?
