• This record comes from PubMed

Correlation between off-axis illumination and apodized phase-contrast: two complementary microscopic phase-imaging modes

. 2008 Sep-Oct ; 13 (5) : 054067.

Language English Country United States Media print

Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't

Microscopic images of biological phase specimens of various optical thickness, acquired under off-axis illumination and apodized/conventional phase-contrast are compared. The luminance profiles in appropriately filtered apodized phase-contrast images compare well with those in the original off-axis illumination images. The two unfiltered image types also yield similar results in terms of quasi-three-dimensional surface (pseudo-relief) rendering, and thus are comparable in terms of the information contents (optical thickness map). However, the overall visual impression is very different as the visual cues to depth structure are present in the off-axis illumination images only. The comparison demonstrated in the present paper was made possible owing to apodization, which substantially reduces the "halo"/shade-off artifacts in the phase-contrast images. The results imply the possibility of combining the off-axis illumination and apodized phase-contrast imaging to examine specimens of medium optical thickness, in which the phase visualization capability of the two imaging modes substantially overlaps (e.g., larger cells or cell clusters).

References provided by Crossref.org

Newest 20 citations...

See more in
Medvik | PubMed

Holography microscopy as an artifact-free alternative to phase-contrast

. 2018 Feb ; 149 (2) : 179-186. [epub] 20171128

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...