The roles of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (p38 MAPK) in aged pig oocytes
Language English Country Japan Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19023183
DOI
10.1262/jrd.20061
PII: JST.JSTAGE/jrd/20061
Knihovny.cz E-resources
- MeSH
- Anthracenes pharmacology MeSH
- Enzyme Inhibitors pharmacology MeSH
- JNK Mitogen-Activated Protein Kinases antagonists & inhibitors metabolism physiology MeSH
- Cells, Cultured MeSH
- Metaphase drug effects physiology MeSH
- p38 Mitogen-Activated Protein Kinases antagonists & inhibitors metabolism physiology MeSH
- Oocytes drug effects enzymology metabolism MeSH
- Swine * metabolism physiology MeSH
- Pyrazoles pharmacology MeSH
- Cleavage Stage, Ovum drug effects enzymology metabolism MeSH
- Cellular Senescence physiology MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anthracenes MeSH
- Enzyme Inhibitors MeSH
- JNK Mitogen-Activated Protein Kinases MeSH
- p38 Mitogen-Activated Protein Kinases MeSH
- Pyrazoles MeSH
After reaching metaphase II, in vitro matured oocytes undergo the complex processes referred to as oocyte aging. Under our culture conditions, some aged oocytes remained at the stage of metaphase II, some underwent spontaneous parthenogenetic activation and others underwent cellular death, either through apoptosis (fragmentation) or lysis. We investigated the effect of c-Jun N-terminal kinases (JNK) and p38 Mitogen-activated protein kinase (p38 MAPK) inhibition on pig oocyte aging and the activity of JNK and p38 MAPK during the aging period. Inhibition of JNK protected the oocytes from fragmentation (0% fragmented oocytes under JNK inhibition vs. 26% fragmented oocytes in the control group). Inhibition of p38 MAPK had no effect on fragmentation. Inhibition of JNK also had an influence on spontaneous parthenogenetic activation of aged oocytes. The ratio of activated JNK to total JNK decreased during aging of oocytes. However, exit from MII had no effect on it. The ratio of activated p38 MAPK to total p38 MAPK did not change significantly. The phosphorylated form of JNK is present in fragmented and activated oocytes, while lysed oocytes lack the active form of JNK. Based on our data, we can conclude that JNK plays an active role in fragmentation of pig oocytes and that p38 MAPK is not involved in this process.
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