Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea
Language English Country Great Britain, England Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
19193998
PubMed Central
PMC2635868
DOI
10.1107/s1744309108040578
PII: S1744309108040578
Knihovny.cz E-resources
- MeSH
- Photosystem II Protein Complex analysis chemistry MeSH
- Crystallization MeSH
- Crystallography, X-Ray MeSH
- Recombinant Proteins analysis chemistry MeSH
- Plant Proteins analysis chemistry MeSH
- Spinacia oleracea chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Photosystem II Protein Complex MeSH
- Recombinant Proteins MeSH
- Plant Proteins MeSH
Preliminary X-ray diffraction analysis of the extrinsic PsbP protein of photosystem II from spinach (Spinacia oleracea) was performed using N-terminally His-tagged recombinant PsbP protein overexpressed in Escherichia coli. Recombinant PsbP protein (thrombin-digested recombinant His-tagged PsbP) stored in bis-Tris buffer pH 6.00 was crystallized using the sitting-drop vapour-diffusion technique with PEG 550 MME as a precipitant and zinc sulfate as an additive. SDS-PAGE analysis of a dissolved crystal showed that the crystals did not contain the degradation products of recombinant PsbP protein. PsbP crystals diffracted to 2.06 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 38.68, b = 46.73, c = 88.9 A.
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Balsera, M., Arellano, J. B., Revuelta, J. L., De Las Rivas, J. & Hermoso, J. A. (2005). J. Mol. Biol.350, 1051–1060. PubMed
Barber, J. (2003). Q. Rev. Biophys.36, 71–89. PubMed
Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760–763. PubMed
De Las Rivas, J., Balsera, M. & Barber, J. (2004). Trends Plant Sci.9, 18–25. PubMed
Evans, P. R. (1997). Jnt CCP4/ESF–EACBM Newsl. Protein Crystallogr.33, 22–24.
Ifuku, K., Nakatsu, T., Kato, H. & Sato, F. (2003). Acta Cryst. D59, 1462–1463. PubMed
Ifuku, K., Nakatsu, T., Kato, H. & Sato, F. (2004). EMBO Rep.5, 362–367. PubMed PMC
Ifuku, K., Nakatsu, T., Shimamoto, R., Yamamoto, Y., Ishihara, S., Kato, H. & Sato, F. (2005). Photosyn. Res.84, 251–255. PubMed
Ifuku, K., Yamamoto, Y., Ono, T., Ishihara, S. & Sato, F. (2005). Plant Physiol.139, 1175–1184. PubMed PMC
Jansen, T., Rother, C., Steppuhn, J., Reinke, H., Beyreuther, K., Jansson, C., Andersson, B. & Herrmann, R. G. (1987). FEBS Lett.216, 234–240.
Kamiya, N. & Shen, J. R. (2003). Proc. Natl Acad. Sci. USA, 100, 98–102.
Laemmli, U. K. (1970). Nature (London), 227, 680–685. PubMed
Leslie, A. G. W. (1999). Acta Cryst. D55, 1696–1702. PubMed
Loll, B., Cern, J., Saenger, W., Zouni, A. & Biesiadka, J. (2005). Nature (London), 438, 1040–1044. PubMed
Seidler, A. (1996). Biochim. Biophys. Acta, 1277, 35–60. PubMed
Shen, J. R. & Inoue, Y. (1993). Biochemistry, 32, 1825–1832. PubMed
Suorsa, M., Sirpio, S., Allahverdiyeva, Y., Paakkarinen, V., Mamedov, F., Styring, S. & Aro, E. (2006). J. Biol. Chem.281, 145–150. PubMed
Yi, X. P., Hargett, S. R., Liu, H., Frankel, L. K. & Bricker, T. M. (2007). J. Biol. Chem.282, 24833–24841. PubMed
Zouni, A., Witt, H. T., Kern, J., Fromme, P., Krauss, N., Saenger, W. & Orth, P. (2001). Nature (London), 409, 739–743. PubMed
Resonance assignment of PsbP: an extrinsic protein from photosystem II of Spinacia oleracea
