Adenosine A(1), A(2a), A(2b), and A(3) receptors in hematopoiesis. 2. Expression of receptor mRNA in resting and lipopolysaccharide-activated mouse RAW 264.7 macrophages
Language English Country Czech Republic Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19249906
DOI
10.33549/physiolres.931724
PII: 1724
Knihovny.cz E-resources
- MeSH
- Macrophage Activation * drug effects genetics MeSH
- Cell Line MeSH
- Time Factors MeSH
- Hematopoiesis * drug effects genetics MeSH
- Lipopolysaccharides pharmacology MeSH
- Macrophages drug effects metabolism MeSH
- RNA, Messenger metabolism MeSH
- Mice MeSH
- Polymerase Chain Reaction MeSH
- Receptors, Purinergic P1 drug effects genetics MeSH
- Receptor, Adenosine A1 genetics MeSH
- Receptor, Adenosine A2A genetics MeSH
- Receptor, Adenosine A2B genetics MeSH
- Receptor, Adenosine A3 genetics MeSH
- Gene Expression Regulation MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Lipopolysaccharides MeSH
- RNA, Messenger MeSH
- Receptors, Purinergic P1 MeSH
- Receptor, Adenosine A1 MeSH
- Receptor, Adenosine A2A MeSH
- Receptor, Adenosine A2B MeSH
- Receptor, Adenosine A3 MeSH
Expression of mRNA for adenosine receptor subtypes A(1), A(2a), A(2b), and A(3) in normal and lipopolysaccharide (LPS)-activated murine RAW 264.7 macrophages has been investigated using the method of quantitative real-time polymerase chain reaction. The results have shown a very low, unquantifiable expression of adenosine A(1) receptor mRNA in both normal and LPS-activated macrophages. The other three adenosine receptor mRNAs have been found to be expressed at various but always quantifiable levels. Activation of the macrophages by LPS induced upregulation of the expression of adenosine receptor A(2a) and A(2b) mRNA, whereas the expression of adenosine receptor A(3) mRNA was downregulated. Unstimulated macrophages exhibited a high expression of the A(2b) adenosine receptor mRNA. The findings are discussed from the point of view of the antiinflammatory and hematopoiesis-stimulating roles of the adenosine receptor signaling.
References provided by Crossref.org
The role of adenosine receptor agonists in regulation of hematopoiesis
