The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19362170
DOI
10.1016/j.intimp.2009.04.005
PII: S1567-5769(09)00136-2
Knihovny.cz E-resources
- MeSH
- Cell Extracts MeSH
- Cell Line MeSH
- Down-Regulation MeSH
- Nitrites metabolism MeSH
- Ethylenediamines MeSH
- Lipids chemistry MeSH
- Lipopolysaccharides metabolism MeSH
- Macrophages drug effects enzymology pathology MeSH
- Mice MeSH
- Histamine H1 Antagonists, Non-Sedating chemistry pharmacology MeSH
- Nitric Oxide metabolism MeSH
- Sulfanilamides MeSH
- Nitric Oxide Synthase Type II metabolism MeSH
- Chromatography, High Pressure Liquid MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cell Extracts MeSH
- Nitrites MeSH
- Ethylenediamines MeSH
- Griess reagent MeSH Browser
- Lipids MeSH
- Lipopolysaccharides MeSH
- Histamine H1 Antagonists, Non-Sedating MeSH
- Nos2 protein, mouse MeSH Browser
- Nitric Oxide MeSH
- Sulfanilamides MeSH
- Nitric Oxide Synthase Type II MeSH
H1-antihistamines are known to be important modulators of inflammatory response. However, the information about the influence of these drugs on reactive nitrogen species generation is still controversial. The main aim of the present study was to investigate the effects of selected H1-antihistamines on nitric oxide production by lipopolysaccharide-stimulated murine macrophages RAW 264.7, measured as changes in inducible nitric oxide synthase (iNOS) protein expression in cell lysates by Western blotting and nitrite formation in cell supernatants using the Griess reaction. In pharmacological non-toxic concentrations, H1-antihistamines significantly inhibited nitrite accumulation that was not caused by the scavenging ability of drugs against nitric oxide, measured amperometrically. The degree of inhibition of nitrite accumulation positively correlated with the degree of tested lipophilicity, measured by reversed-phase thin layer chromatography. Furthermore, H1-antihistamines differentially modulated the iNOS protein expression. In conclusion, as was shown in this study, the modulation of nitric oxide production could be caused by the downregulation of iNOS protein expression and/or the iNOS protein activity.
References provided by Crossref.org
Modulation of metabolic activity of phagocytes by antihistamines
